Non-motile organisms grow only along the stab line and leave the surrounding medium clear. Motility Test Medium with Triphenyltetrazolium Chloride (TTC) is used in the same manner, however, TTC adds visual enhancement of bacterial growth. Ingredients per liter of deionized water:* Final p H 7.3 +/- 0.3 at 25ÂșC. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed.

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Motile organisms produce a pink color that diffuses from the stab line.

Organisms that are non-motile produce a pinkish-red pigment that is confined to the stab line.

Organisms are stabbed into the medium which is then incubated for a 24 hour period.

Motile organisms extend from the stab line and produce turbidity or cloudiness throughout the medium.

Hardy Diagnostics Motility Test Medium is a modification of the formula employed by Tittsler and Sandholzer.

Small amounts of agar and gelatin are added to provide a semi-solid medium which allows macroscopic examination of bacterial motility.

Specimen Collection: Specimen collection is not applicable since this medium is not intended for primary isolation from clinical specimens.

Motility Test Medium with and without Triphenyltetrazolium Chloride (TTC) is recommended for the detection of motility in gram-negative enteric bacilli.

TTC is added to enhance visualization of bacterial growth.

TTC is a colorless dye that, when reduced by bacterial cells, produces formazan, an insoluble red pigment. Color Atlas and Textbook of Diagnostic Microbiology, J.

The red color forms only in the area of bacterial growth.